Chlorophyll A is responsible for absorbing energy from light, and ultimately donating that energy in the form of electrons to neighboring photosystems I and II. If you’ve ever cracked open a biology book or watched a Planet Earth or Discovery Channel special on plants you’ve surely heard of this molecule. The solution to this is by extracting, and measuring, a pigment found in these cells called chlorophyll A. Clumps of bacteria form during log and stationary phase and cause inaccurate results by shielding other bacteria from detection. Since we work on filamentous bacteria, classic techniques, such as measuring the turbidity of our cell culture (such as by measuring OD 720) is quite difficult. Perhaps the very first skill learned in the Summers lab (after axenic culturing technique) is the chlorophyll A extraction.
0 kommentar(er)
